Electrochemical approach for the in situ synthesis of a poly(sulfosalicylic acid)/nickel oxide nanoparticles/MCM-41 nanocomposite for guanine detection

Document Type : Original Research Paper

Authors

University of Guilan

10.22111/cnmst.2025.51292.1258

Abstract

Guanine, a purine base in deoxyribonucleic acid (DNA), has a low redox potential, making it susceptible to oxidation and contributing to DNA damage and mutations. In the current study, a new polymer nanocomposite consisting of nickel oxide-Mobile crystalline material-41 (NiO-MCM-41) nanocomposites and poly(5-sulfosalicylic acid) (PSSA) was prepared by electropolymerization for modification of the graphite/glassy carbon electrode (Gr/GCE) surface and sensitive measurement of the guanine. The synthesized nanocomposite was identified and confirmed by FE-SEM, EDX and FT-IR techniques. The electrochemical behavior of guanine on the surface of the PSSA/NiO-MCM-41/Gr/GCE was analyzed in optimal conditions using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The proposed modified electrode achieved a satisfactory dynamic range between the anodic peak current and the concentration of guanine, with a linear range of 0.001–36 µM, a sensitivity of 0.977 µA/µM, and the detection limit (LOD) was determined to be 0.5 nM (signal/noise = 3). This sensor was successfully used in the determination of guanine in the real sample of fish sperm’s DNA.

Keywords

Challenges in Nano and Micro Scale Science and Technology
Volume 11, Issue 2
November 2023
Pages 40-51

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